Dianfeng Chemical
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1x 2 Mercaptoethanol applications properties synthesis safety
A Brief Description of 1x + 2 Mercaptoethanol
Application of 1x + 2 Mercaptoethanol
1x + 2 Mercaptoethanol has important applications in many fields. In biochemical experiments, it is often used as a key reagent for protein and nucleic acid research. In protein analysis, it can open disulfide bonds within or between protein molecules, allowing protein subunits to be separated, facilitating in-depth investigation of protein structure and function. For example, in protein electrophoresis experiments, the addition of this reagent ensures that the protein swims in monomer form and its molecular weight is accurately determined. In the process of nucleic acid extraction, 1x + 2 mercaptoethanol helps to protect nucleic acid from oxidative damage, maintain nucleic acid integrity, and improve extraction quality. In the field of cell culture, appropriate addition of 1x + 2 mercaptoethanol can improve the cell growth environment and promote the proliferation and survival of some cell lines, especially for some cells sensitive to oxidative stress.
Properties of di, 1x + 2 mercaptoethanol
1x + 2 mercaptoethanol has unique physical and chemical properties. From the physical properties, it is a colorless and transparent liquid with a special odor. It can be miscible with various organic solvents such as water, alcohol, and ether, and has good solubility. In terms of chemical properties, thiol (-SH) is its reactive group, giving it strong reductivity. The reagent is easily oxidized and can be gradually oxidized to disulfide in air. This reductivity enables it to react with many oxygenated compounds and is used as a reducing agent in organic synthesis. It also has a certain acidity, can neutralize with bases, and can adjust the pH of the reaction in a specific reaction system.
Synthesis of tri, 1x + 2 mercaptoethanol
Synthesis methods of 1x + 2 mercaptoethanol are diverse. The classical method is to use ethylene oxide and hydrogen sulfide as raw materials and carry out the reaction under the action of catalyst. The specific reaction process is as follows: under suitable temperature and pressure, the catalyst prompts the ring-opening addition reaction of ethylene oxide and hydrogen sulfide to generate 1x + 2 mercaptoethanol. Another method is to use chloroethanol and sodium sulfide as raw materials and prepare by nucleophilic substitution reaction. In the reaction, the chlorine atoms in chloroethanol are replaced by sulfur ions to form the target product. In addition, there are some improved synthesis processes, such as the use of phase transfer catalysts, etc., to improve the reaction efficiency and product purity, optimize the synthesis conditions, reduce production costs and environmental pollution.
Safety precautions for tetra-, 1x + 2 mercaptoethanol
1x + 2 mercaptoethanol has certain dangers, and special attention should be paid to safety when using. Because of its volatility, inhaling the vapors evaporated by this reagent may irritate the respiratory tract, causing symptoms such as cough and asthma, and even damage the respiratory system in severe cases. Skin contact can cause irritation, redness and swelling. If the skin is damaged, it may be absorbed into the body, causing greater harm to the body. Eye contact can strongly irritate the eye tissue, with the risk of causing visual impairment. Therefore, the operation should be carried out in a well-ventilated environment, preferably in a fume hood. Operators need to wear suitable protective equipment, such as protective glasses, gloves and protective clothing. In case of accidental contact, the contact area should be washed with a lot of water immediately, and medical attention should be sought in serious cases. At the same time, the reagent should be stored in a cool and ventilated warehouse, away from fire and heat sources, and avoid mixed storage with oxidants to prevent dangerous reactions.
Application of 1x + 2 Mercaptoethanol
1x + 2 Mercaptoethanol has important applications in many fields. In biochemical experiments, it is often used as a key reagent for protein and nucleic acid research. In protein analysis, it can open disulfide bonds within or between protein molecules, allowing protein subunits to be separated, facilitating in-depth investigation of protein structure and function. For example, in protein electrophoresis experiments, the addition of this reagent ensures that the protein swims in monomer form and its molecular weight is accurately determined. In the process of nucleic acid extraction, 1x + 2 mercaptoethanol helps to protect nucleic acid from oxidative damage, maintain nucleic acid integrity, and improve extraction quality. In the field of cell culture, appropriate addition of 1x + 2 mercaptoethanol can improve the cell growth environment and promote the proliferation and survival of some cell lines, especially for some cells sensitive to oxidative stress.
Properties of di, 1x + 2 mercaptoethanol
1x + 2 mercaptoethanol has unique physical and chemical properties. From the physical properties, it is a colorless and transparent liquid with a special odor. It can be miscible with various organic solvents such as water, alcohol, and ether, and has good solubility. In terms of chemical properties, thiol (-SH) is its reactive group, giving it strong reductivity. The reagent is easily oxidized and can be gradually oxidized to disulfide in air. This reductivity enables it to react with many oxygenated compounds and is used as a reducing agent in organic synthesis. It also has a certain acidity, can neutralize with bases, and can adjust the pH of the reaction in a specific reaction system.
Synthesis of tri, 1x + 2 mercaptoethanol
Synthesis methods of 1x + 2 mercaptoethanol are diverse. The classical method is to use ethylene oxide and hydrogen sulfide as raw materials and carry out the reaction under the action of catalyst. The specific reaction process is as follows: under suitable temperature and pressure, the catalyst prompts the ring-opening addition reaction of ethylene oxide and hydrogen sulfide to generate 1x + 2 mercaptoethanol. Another method is to use chloroethanol and sodium sulfide as raw materials and prepare by nucleophilic substitution reaction. In the reaction, the chlorine atoms in chloroethanol are replaced by sulfur ions to form the target product. In addition, there are some improved synthesis processes, such as the use of phase transfer catalysts, etc., to improve the reaction efficiency and product purity, optimize the synthesis conditions, reduce production costs and environmental pollution.
Safety precautions for tetra-, 1x + 2 mercaptoethanol
1x + 2 mercaptoethanol has certain dangers, and special attention should be paid to safety when using. Because of its volatility, inhaling the vapors evaporated by this reagent may irritate the respiratory tract, causing symptoms such as cough and asthma, and even damage the respiratory system in severe cases. Skin contact can cause irritation, redness and swelling. If the skin is damaged, it may be absorbed into the body, causing greater harm to the body. Eye contact can strongly irritate the eye tissue, with the risk of causing visual impairment. Therefore, the operation should be carried out in a well-ventilated environment, preferably in a fume hood. Operators need to wear suitable protective equipment, such as protective glasses, gloves and protective clothing. In case of accidental contact, the contact area should be washed with a lot of water immediately, and medical attention should be sought in serious cases. At the same time, the reagent should be stored in a cool and ventilated warehouse, away from fire and heat sources, and avoid mixed storage with oxidants to prevent dangerous reactions.
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