Dianfeng Chemical
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2 Mercaptoethanol Agglutination Test
Dimercaptoethanol agglutination test
1. INTRODUCTION
The diagnosis of husband's disease, there are all kinds of test methods, and now the main points of dimercaptoethanol agglutination test are described in detail.
II. Principle
The basis of this test is that dimercaptoethanol can break certain antibody structures. Antibodies that cover the body have their specific conformation and chemical structure. Dimercaptoethanol has strong reductivity and can break specific chemical bonds in antibody molecules, especially disulfide bonds. When the disulfide bond is broken, the spatial structure of the antibody changes and its activity is also different. With this characteristic, the reaction of relevant antigens and antibodies can be revealed.
3. Operation process
1. ** Specimen preparation **: Select a suitable specimen, such as serum. When collecting blood, follow the rules of sterility to make the blood self-coagulate, then centrifuge and remove it, and store it under suitable conditions to prevent its deterioration.
2. ** Reagent configuration **: Prepare dimercaptoethanol reagent, according to the precise ratio, with a suitable solvent. Pay attention to the ambient temperature and humidity when preparing, and stir well to ensure uniform reagent concentration.
3. ** Test steps **: In the well of the clean reaction plate, add serum specimen, dimercaptoethanol reagent and known antigens in sequence. Use a pipette to control the amount accurately. Then mix well, set it in a suitable incubation environment, such as an incubator, and observe it regularly.
IV. Results Judgment
After incubation, the appearance of agglutination in the observation pores. If there is obvious agglutination, such as granular or flocculent, and the distribution is uniform, this is a positive sign, indicating that there is an antibody specifically bound to the antigen in the serum, and it is still active after treatment with dimercaptoethanol; if the liquid in the pores is clear and there is no agglutination, it is negative, and there is no corresponding active antibody in the serum, or the amount of antibody is small, which is not enough to form agglutination.
V. Application
This test is widely used in the diagnosis of diseases. For example, the diagnosis of certain viral infections can help to determine whether the pathogen is infected or not. And the results can provide evidence for the treatment policy. If positive, it can push the progress of the epidemic and help the doctor choose the right treatment, or drug treatment, or other methods to help the patient recover.
1. INTRODUCTION
The diagnosis of husband's disease, there are all kinds of test methods, and now the main points of dimercaptoethanol agglutination test are described in detail.
II. Principle
The basis of this test is that dimercaptoethanol can break certain antibody structures. Antibodies that cover the body have their specific conformation and chemical structure. Dimercaptoethanol has strong reductivity and can break specific chemical bonds in antibody molecules, especially disulfide bonds. When the disulfide bond is broken, the spatial structure of the antibody changes and its activity is also different. With this characteristic, the reaction of relevant antigens and antibodies can be revealed.
3. Operation process
1. ** Specimen preparation **: Select a suitable specimen, such as serum. When collecting blood, follow the rules of sterility to make the blood self-coagulate, then centrifuge and remove it, and store it under suitable conditions to prevent its deterioration.
2. ** Reagent configuration **: Prepare dimercaptoethanol reagent, according to the precise ratio, with a suitable solvent. Pay attention to the ambient temperature and humidity when preparing, and stir well to ensure uniform reagent concentration.
3. ** Test steps **: In the well of the clean reaction plate, add serum specimen, dimercaptoethanol reagent and known antigens in sequence. Use a pipette to control the amount accurately. Then mix well, set it in a suitable incubation environment, such as an incubator, and observe it regularly.
IV. Results Judgment
After incubation, the appearance of agglutination in the observation pores. If there is obvious agglutination, such as granular or flocculent, and the distribution is uniform, this is a positive sign, indicating that there is an antibody specifically bound to the antigen in the serum, and it is still active after treatment with dimercaptoethanol; if the liquid in the pores is clear and there is no agglutination, it is negative, and there is no corresponding active antibody in the serum, or the amount of antibody is small, which is not enough to form agglutination.
V. Application
This test is widely used in the diagnosis of diseases. For example, the diagnosis of certain viral infections can help to determine whether the pathogen is infected or not. And the results can provide evidence for the treatment policy. If positive, it can push the progress of the epidemic and help the doctor choose the right treatment, or drug treatment, or other methods to help the patient recover.
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