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Hydrogen Sulfide Test Principle
Principle of hydrogen sulfide test
For hydrogen sulfide testers, it is also possible to test the ability of microorganisms to metabolize hydrogen sulfide. The reason is as follows:
Microorganisms behave differently during growth and metabolism. Some can decompose specific sulfur-containing compounds, such as cystine and cysteine. Such amino acids are rich in sulfur. When microorganisms have corresponding enzyme systems, such as cystinase, they can catalyze the decomposition of sulfur-containing amino acids such as cystine.
When decomposed, sulfur is released and combined with free hydrogen in the environment to form hydrogen sulfide gas. To test the formation of this hydrogen sulfide, lead acetate or ferrous sulfate are often used as indicators. When hydrogen sulfide encounters lead acetate, it is synthesized into lead sulfide. This is a black precipitation, which is black and obvious and easy to identify. When it encounters ferrous sulfate, it becomes ferrous sulfide, also black, to show the production of hydrogen sulfide.
From this perspective, in the medium, if black precipitation is seen, it can be seen that this microorganism has the ability to produce hydrogen sulfide, which is the principle of hydrogen sulfide testing.
For hydrogen sulfide testers, it is also possible to test the ability of microorganisms to metabolize hydrogen sulfide. The reason is as follows:
Microorganisms behave differently during growth and metabolism. Some can decompose specific sulfur-containing compounds, such as cystine and cysteine. Such amino acids are rich in sulfur. When microorganisms have corresponding enzyme systems, such as cystinase, they can catalyze the decomposition of sulfur-containing amino acids such as cystine.
When decomposed, sulfur is released and combined with free hydrogen in the environment to form hydrogen sulfide gas. To test the formation of this hydrogen sulfide, lead acetate or ferrous sulfate are often used as indicators. When hydrogen sulfide encounters lead acetate, it is synthesized into lead sulfide. This is a black precipitation, which is black and obvious and easy to identify. When it encounters ferrous sulfate, it becomes ferrous sulfide, also black, to show the production of hydrogen sulfide.
From this perspective, in the medium, if black precipitation is seen, it can be seen that this microorganism has the ability to produce hydrogen sulfide, which is the principle of hydrogen sulfide testing.
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